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Protocols

Immunopurification protocol for the pulldown of endogenous RNA-binding proteins ("RIP") and co-purification of (associated) RNA molecules from human cell extracts. RNA prepared according to this protocol can be analyzed using microarrays or (possibly) sequencing techniques, allowing the identification of the RNA targets of the RBP under study.

This protocol has been optimized for the immunopurification of endogenous human PUM1 and PUM2 proteins from HeLa S3 cells.

Protocol by Alessia Galgano.

References:

Error fetching PMID 18776931:
Error fetching PMID 21431753:
  1. Error fetching PMID 18776931: [Original_article]
  2. Error fetching PMID 21431753: [Method]
All Medline abstracts: PubMed HubMed



Immunopurification protocol for the pulldown of tagged ribosomal proteins and the co-purification of (associated) RNA molecules from yeast cell extracts. RNA prepared according to this protocol can be analyzed using microarrays or (possibly) sequencing techniques.

This protocol has been optimized for the study of yeast ribosomes through the immunopurification of the large ribsomal subunit protein Rpl16a.

Protocol by Regula Halbeisen.

References:

Error fetching PMID 19419242:
Error fetching PMID 19398006:
  1. Error fetching PMID 19419242: [Original_article]
  2. Error fetching PMID 19398006: [Method]
All Medline abstracts: PubMed HubMed